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Chondroitin sulfate perlecan enhances collagen fibril formation. We first engineered BglG derivatives with a specific protease (factor Xa) cleavage site that allowed asymmetric splitting of each prephosphorylated protein to well defined peptides, of which only one was labeled by radioactive phosphate. Phosphorylation of actin Tyr-53 inhibits filament nucleation and elongation and destabilizes filaments. Heated NCP, heated HNE, and CMK-complexed HNE all increased transendothelial albumin transfer. Bile secretion by the rat liver during synchronized regeneration. In our opinion, a mere wait-and-see strategy completely without any treatment can hardly be recommended. On relapse, radiotherapy alone or in combination with short courses of chemotherapy was performed. We have demonstrated the alteration of the localization of ecto-ATPase activity in human neutrophils after stimulation with phorbol myristate acetate or N-formylmethionyl-leucyl-phenylalanine using a cerium-based cytochemical method. Studies of porcine reproductive and respiratory syndrome (PRRS) virus infection in avian species. Soil respiration rates were significantly higher in burned sites than in controls.
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Distinct Ras effector pathways are involved in Fc epsilon R1 regulation of the transcriptional activity of Elk-1 and NFAT in mast cells. Micropatterned substrates were prepared by fabricating poly(ethylene glycol) (PEG) hydrogel microstructures on alumina membranes with 200 nm nanopores using photolithography. Two groups were individualized: in the first group (62 women) we obtained at least once, 3 oocytes (288 attempts), in the second group (38 women) we always obtained less than 3 oocytes. Distinct cell uptake mechanisms make PDCs less sensitive to efflux pumps associated with the development of multi-drug resistance. The solution conformation of the recently discovered bi-cyclic, 21 amino acid vasoconstrictor peptide, Endothelin I, has been examined by 1H-NMR in deuterated dimethyl sulphoxide. Familial aggregation of inflammatory bowel disease: a population-based study in South Limburg, The Netherlands.
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This review discusses select current applications of fluorescent in situ hybridization (FISH) which may be of utility for the average clinical cytogenetic laboratory. Currently, chronic seizures after brain injury are often poorly controlled by available antiepileptic drugs. Profile of Professor Katsuya Itahara, Clinical Director of Internal Medicine, Research Institute of Brain Diseases, Tohoku University School of Medicine. Chronic granulomatous disease involving the larynx and bronchus. When alcohol based fixatives are used for fixation, precautions should be taken with the material thus treated, as it may contain viable spores or other pathogens, which are destroyed after 24 hours of formalin treatment. Ultrastructural studies on sporulation in wild-type and white colony mutants of Streptomyces coelicolor. Data were confirmed with facility records, observations and staff interviews. By using time-resolved fluorescence microscopy on a variety of cell types, we have shown that the dual-emission fluorescent pH probe carboxy SNARF-1 binds to cellular components in significant levels.
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During the Cretaceous, notosuchians embodied a second diversification event in terrestrial habitats and eusuchian lineages started diversifying before the end of the Mesozoic. The biomechanical and MR properties can be adjusted to be similar to those of excised porcine aortas by varying the number of freeze-thaw cycles to which the PVA solution is exposed. Abnormalities of vascular morphology and angiogenesis have been described at the macroscopic, histological and molecular levels in the synovial membrane in rheumatoid, seronegative and degenerative arthritis. A multicenter controlled double-blind study with a modified-release formulation. Participating in mobility: People with schizophrenia driving motorized vehicles. G gene-deficient single-round rabies viruses for neuronal circuit analysis. Thus, the improvement in performance after the 10 mg/kg dose of chlorpheniramine can be interpreted in terms of a compensation of performance deficits in the old rats. A dimeric aluminium hydroxide supported by a new disiloxide ligand. Role for Reelin-induced cofilin phosphorylation in the assembly of sympathetic preganglionic neurons in the murine intermediolateral column.
Direct intraspinal injection of the catecholamines epinephrine and norepinephrine, and the alpha-adrenergic agents dexmedetomidine and clonidine, produced a dose-dependent elevation of pain thresholds in the Northern grass frog, Rana pipiens. Paracetamol metabolism in two ethnically different Spanish populations. Inhibited development of Ostertagia ostertagi, Cooperia oncophora, and Nematodirus helvetianus in parasite-free calves grazing fall pastures. The paper presents a method for the qualitative assessment of coupling forces. The alpha beta and gamma delta T cells develop from common T-cell progenitors and the alpha beta/gamma delta lineage commitment process should be viewed in the context of the diverse cell fate decision processes crucial for normal cell differentiation from a single pluiripotent stem cell. A large group of phage receptors compose porin proteins, which are discovered in 32 species of bacteria.
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Cartilage on bone, obtained from bovine shoulder joints, was tested. This study assessed the effects of carbenoxolone on human myocardial conduction and refractoriness. Further evidence that the site of the breakpoint in the major breakpoint cluster region (M-bcr) may be a prognostic indicator. After a median follow-up time of 46 (2-97) months, a multivariate analysis of risk factors for metastases was performed in 138 radically operated on patients with tumors of the extremities. Treated cells demonstrated increased protein levels of p53 and the p53 targets p21 and HDM2.
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This software used known pedigrees in the captive baboon sample and tested the relationship between pairwise kinship and alveolar bone loss data to determine the heritability of alveolar bone loss from periodontal disease. In this protocol, we provide individual steps for utilizing Gateway recombination for cloning as well as detailed instructions for screening targeted ES cell clones. Calcium appears to be the plasma cation which protects factors V and VIII against in vitro loss of activity. A critical overview of the most representative compounds is provided with emphasis on the HDAC inhibitor-based hybrid agents. Bile ptyalism in a patient with sickle cell anemia simulating bronchobiliary fistula.
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Formation of hemichrome during reaction of hemoglobin with polar phosphatidylcholine derivatives. When trpB translation proceeded in the wild type reading frame and terminated at the normal trpB stop codon, trpA polypeptide levels were elevated over the levels observed when translation stopped before or after the natural trpB stop codon. Trace element speciation in biomedical and environmental science has gained increasing attention over the past decade as researchers have begun to realize its importance in toxicological studies. Types of investigation and integration of this dimension in an eventual therapeutic process strictly respecting the needs and specificities of each one will be discussed. Production of dihydrothymine in irradiated DNA correlated with the level of reducing species produced by X-rays, and dihydrothymine was produced preferentially in irradiated single-stranded or denatured DNA as compared to irradiated duplex DNA. The addition of CDDP to 131I-323/A3 could increase the growth inhibition in the CDDP-responsive FMa and OVCAR-3 xenografts, but not in Ov.Pe xenografts.
The latter component is mainly driven by the abduction of the second branchial arch. These two signaling changes confer excitatory effects on the cell in place of the typical inhibition by opioids and are associated with morphine tolerance and dependence. A very remarkable finding is that increased excess area is not always stored in the contact area, but leads to the formation of microbuds (diameter approximately 2 microns). The basal hippurate level was slightly diminished and its decrease in the course of the oral glucose tolerance test was similar to that seen in the volunteers. Specific monovalent cation effects on modification of reovirus infectivity by chymotrypsin digestion in vitro. To determine if IFN-gamma is necessary in reexposure histoplasmosis, GKO and WT mice were inoculated with 10(4) yeasts intranasally and given amphotericin B for 3 weeks.
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Ten patients with elective percutaneous transluminal coronary angioplasty served as a control group. The general aspects and newer applications of two-dimensional, contrast, and doppler echocardiography in the assessment of congenital heart diseases are reviewed. The isovolumic bulge in the ischemic area was more pronounced at an end-diastolic pressure of 5 mmHg than it was at an end-diastolic pressure of 15 mmHg. Parathyroid hormone-induced calcium efflux from cultured bone is mediated by protein kinase C translocation. The effect of cattle manure cultivation on moisture content and survival of Escherichia coli. Mutations that change the immunological subtype of hepatitis B virus surface antigen and distinguish between antigenic and immunogenic determination.
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A number of CD3-positive T-lymphocytes were found around foam cells expressing Fas/APO 1. All patients were examined by ultrasound before laparoscopy or/and laparotomy. In CSE, the weak coupling Hamiltonian allows development of antiphase coherences which, in the presence of the line broadening due to the imaging gradients, result in signal loss. The role of Erlebnistypus personality features were investigated in 40 healthy subjects, as defined by the Rorschach Comprehensive System by Exner, in determining accuracy in recollecting acute tonic experimental pain, induced by the Cold Water Pressor Test. Use of different definitions of biochemical failure after external beam radiotherapy changes conclusions about relative treatment efficacy for localized prostate cancer. As a result of their slow reorientation in solution, these assemblies have been resistant to the widely employed solution NMR approaches.